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pmt flag relish  (Addgene inc)


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    Structured Review

    Addgene inc pmt flag relish
    Pmt Flag Relish, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+flag+relish/pMT-Flag-RELISH+(Plasmid+%2363750)/pmc12805896-106-6-7
    Average 94 stars, based on 1 article reviews
    pmt flag relish - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid, #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Injection:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Transgenic Assay:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Generated:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Polymerase Chain Reaction:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Amplification:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid, #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Sequencing:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid, #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Clone Assay:

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTC ACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924 ; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGAT GACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGA TGACC) from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid, #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: This plasmid was injected into w1118 embryos (Rainbow Transgenic Flies). w1118 ; UAS-HDAC6-HA transgenic reporter flies were generated by PCR amplification of HDAC6 coding sequence with specific primers (F: AACAGATCTGCGGCCGCGGCTCGAGGGTACATGAGCCCGCCCATCGTCACGC, R: GGTTCCTTCACAAAGATCCTCTAGAGTCAAGCGTAATCTGGAACATCGTA) from UGO01255 (DGRC Stock 1663924; https://dgrc.bio.indiana.edu//stock/1663924; RRID: DGRC_1663924), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies). w1118 ; UAS-Relish-V5 transgenic reporter flies were generated by PCR amplification of Relish coding sequence with specific primers (F: AAGAGAACTCTGAATAGGGAATTGGGAATTCGACTACAAGGATGACGATGACAAA, R: GGTTCCTTCACAAAGATCCTCTAGAGGTACCTCAATGGTGATGGTGATGATGACC) from pMT-Flag-RELISH (Addgene plasmid #63750; http://n2t.net/addgene:63750; RRID: Addgene_63750), and then cloned into a pUASt plasmid. .. This plasmid was injected into w1118 ; attp40 embryos (Rainbow Transgenic Flies).

    Article Title: NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila
    Article Snippet: .. Relish coding sequence was amplified from pMT-Flag-RELISH (Addgene plasmid # 63750; http://n2t.net/addgene:63750 ; RRID: Addgene_63750), and then cloned in the pAC5.1 V5-His plasmid (pAC5.1-Relish-V5). ..



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